Highlights
- Red and near-infrared PBM consistently influence mitochondrial ATP production.
- Cytochrome c oxidase and ATP synthase are key photobiological targets.
- ATP increases depend on wavelength, fluence, pulse structure, and tissue type.
- Both in vitro and in vivo models demonstrate ATP enhancement.
- Non-thermal mechanisms predominate.
Background
ATP synthesis is central to PBM’s biological effects. Numerous experimental studies have examined whether red and infrared light can increase ATP levels in cells and tissues, providing mechanistic support for PBM in healing, neuroprotection, and metabolic modulation.
Key Findings
Across preclinical and cellular studies:
- Red (≈630–660 nm) and NIR (≈808–1064 nm) irradiation increased ATP levels in brain, muscle, heart, lymphocytes, and wound tissue.
- Pulsed NIR delivery often produced stronger ATP responses than continuous wave.
- ATP increases were associated with improved mitochondrial membrane potential, gene expression of ETC components, and enhanced oxidative phosphorylation.
- Excessive fluence or power density reduced or reversed ATP gains, highlighting biphasic dose–response behavior.
Takeaway
The literature strongly supports that red and near-infrared photobiomodulation can enhance ATP production through multiple mitochondrial mechanisms. These effects are dose- and parameter-dependent, reinforcing the importance of optimized PBM protocols for therapeutic use.
References
Amat A, Rigau J, Nicolau R, Aalders M, Rosa Fenoll M, van Gemer M, et al. Effect of red and near-infrared laser light on adenosine triphosphate (ATP) in the luciferine–luciferase reaction. Journal of Photochemistry and Photobiology A: Chemistry, 2004;168(1-2):59–65. doi:10.1016/j.jphotochem.2004.05.024
Benedicenti S, Pepe IM, Angiero F, Benedicenti A. Intracellular ATP Level Increases in Lymphocytes Irradiated with Infrared Laser Light of Wavelength 904 nm. Photomedicine and Laser Surgery. 2008;26(5):451–453. doi:10.1089/pho.2007.2218
Keshri GK, Gupta A, Yadav A, Sharma SK, Singh SB. Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats. PLoS One. 2016;11(11):e0166705. doi: 10.1371/journal.pone.0166705.
Lapchak PA, De Taboada L. Transcranial near infrared laser treatment (NILT) increases cortical adenosine-5′-triphosphate (ATP) content following embolic strokes in rabbits. Brain research. 2010; 1306:100–105. doi: 10.1016/j.brainres.2009.10.022
Masha RT, Houreld NN, Abrahamse H. Low-Intensity Laser Irradiation at 660 nm Stimulates Transcription of Genes Involved in the Electron Transport Chain. Photomedicine and Laser Surgery. 2013;31(2):47–53. doi:10.1089/pho.2012.3369
Mochizuki-Oda N, Kataoka Y, Cui Y, Yamada H, Heya M, Awazu K. Effects of near-infra-red laser irradiation on adenosine triphosphate and adenosine diphosphate contents of rat brain tissue. Neuroscience Letters. 2002;323(3):207–210. doi:10.1016/s0304-3940(02)00159-3
Oron U, Yaakobi T, Oron A, Mordechovitz D, Shofti R, Hayam G, et al. Low-energy laser irradiation reduces formation of scar tissue after myocardial infarction in rats and dogs. Circulation. 2001;103(2):296–301. doi:10.1161/01.cir.103.2.296
Ravera S, Ferrando S, Agas D, De Angelis N, Raffetto M, et al. 1064 nm Nd:YAG laser light affect transmembrane mitochondria respiratory chain complexes. Journal of Biophotonics. 2019:e201900101. doi:10.1002/jbio.201900101
Silva LM, Silva CA, Silva Ad, Vieira RP, Mesquita-Ferrari RA, Cogo JC, Zamuner SR. Photobiomodulation Protects and Promotes Differentiation of C2C12 Myoblast Cells Exposed to Snake Venom. PLoS One. 2016;11(4):e0152890. doi: 10.1371/journal.pone.0152890.
Wang L, Hu L, Grygorczyk R, Shen X, Schwarz W. Modulation of extracellular ATP content of mast cells and DRG neurons by irradiation: studies on underlying mechanism of low-level-laser therapy. Mediators of inflammation. 2015:630361. doi:10.1155/2015/630361
Wang L, Sikora J, Hu L, Shen X, Grygorczyk R, Schwarz W. ATP release from mast cells by physical stimulation: a putative early step in activation of acupuncture points. Evidence-based complementary and alternative medicine: eCAM. 2013:350949. doi:10.1155/2013/350949